Offline Internship Courses

Duration: 1 Month
Mode: Ofline
Certification Provided

MOLECULAR BIOLOGY


  • 16S/18S/ITS Gene Sequencing (SangerMethod)
  • Next Generation Sequencing (NGS): Metagenomics, mRNA, Exome, Genome, Transcriptomics
  • Primer Designing and Synthesis
  • Real-Time PCR Analysis
  • Protein/Enzyme/Peptide Purification
  • DNA and RNA Isolation
  • Agarose Gel Electrophoresis & SDS-PAGE Analysis
  • Western Blotting

IMMUNOLOGY


  • Agglutination Tests (Latex, Co-aggregation)
  • Enzyme Immunoassays
  • Complement Fixation
  • Precipitation Tests
  • Immunoblotting
  • Bacteria & Fungi Detection (in biological samples)
  • Detection of Hormones, Drugs, Proteins, Vitamins, Tumor Biomarkers
  • Hepatic/RenalMarkers Profiling (Laboratory Animals)
  • Hematological Analysis

HERBAL TECHNOLOGY


  • Crude Phytocompounds Extraction (Maceration, Soxhlet)
  • TLC Analysis
  • Phytochemicals Analysis
  • Column Chromatography (Compound purification)
  • Herbal Cream Formulation
  • Herbal Lipstick Formulation
  • Herbal Joint Spray Formulation
  • Herbal Dye Formulation
  • Herbal Nasal Inhaler Formulation

HISTOPATHOLOGY


  • Paraffin blocks preparation and Hematoxylin and eosin (H&E) staining
  • Immunohistochemistry Analysis (IHC)
  • Negative Staining
  • TRAP Staining
  • Toluidine blue staining

MARINE BIOTECHNOLOGY


  • Bioluminescence bacteria isolation and characterization
  • Isolation of endophytes from sea weed, fish and other aquatic plants
  • Bioactive secondary metabolites production, optimization, and characterization
  • Anti-cancer enzyme production using marine bacteria
  • Biopolymer extraction from seaweeds

NANOTECHNOLOGY


  • Green Synthesis (Silver, Copper, Zinc, Titanium, Iron, Zeolite, Mesoporous Silica nanoparticles)
  • Chemical Synthesis (Carbon nanotubes, Gold nanoparticles, Ceramic nanoparticles, Graphene, Fullerenes, Metallic & Polymeric Nanoparticles)
  • Chitosan-based Thin Film Preparation
  • Drug-loaded Hydrogel Synthesis
  • Cyclodextrin Nanoparticles (Fortargeted drug delivery)
  • Micro/Nano-emulsion Synthesis & Optimization (For targeted drug delivery)

ANIMAL CELL CULTURING


  • MTT Assay (Anti-cancer, Anti-inflammatory, Cytotoxicity)
  • Cell Lines: MCF7, HepG2, A375, A431, A549, HeLa, SH-SY5Y, L929 MitochondrialMembrane Potential
  • Mitochondrial Membrane Potential
  • Flow Cytometry (Apoptosis)
  • Gene Expression Profiling (Cancer models)
  • Cell Cycle Analysis
  • Oxidative Stress & ROS Assays
  • Wound Healing (Scratch assaywith L929)
  • Immunohistochemistry Analysis (IHC)
  • Negative Staining
  • TRAP Staining
  • Toluidine blue staining

PHARMACOLOGY


  • Drug-like Lead Compound Screening
  • Acute & Chronic Toxicity Testing
  • IC50, LD50, EC50 Prediction
  • In-Vitro Anti-bacterial Activity (Agar well & Disc diffusion)
  • In-Vitro Anti-oxidant Activity (DPPH, FRAP, ABTS, H2O2)
  • In-Vitro Anti-inflammatory Activity (Protein denaturation inhibition)
  • In-Vitro Anti-platelet Activity
  • Antibiotic Susceptibility Testing
  • API Quality Checking (By HPLC)